| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: invention relates to biotechnology, specifically to a method of producing a version of glycolipid acyltransferase enzyme, which involves: (a) selection of an initial enzyme which is glycolipid acyltransferase, where the enzyme contains amino acid motif GDSX, where X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modification of one or more amino acids to obtain a glycolipid acyltransferase version; (c) testing the glycolipid acyltransferase version on transferase activity, and optionally hydrolytic activity with respect to a galactolipid substrate and, optionally, a phospholipid substrate and/or, optionally, triglyceride substrate; (d) selection of the version of the enzyme with high activity with respect to galactolipids compared to the initial enzyme; and, optionally, (e) obtaining a large amount of the version of the enzyme. The invention also relates to versions of the lipidacyltransferase enzyme, where the enzyme contains amino acid motif GDSX, where X is one or more of the following amino acid residues L, A, V, T, F, Y, H, Q, T, N, M or S, and where, compared to the initial sequence, the version of the enzyme contains one or more amino acid modifications. ^ EFFECT: obtaining versions of an enzyme with high transferase activity compared to the initial enzyme. ^ 36 cl, 61 dwg, 14 tbl, 10 ex |
