摘要 |
<p>Disclosed are processes making isoforskolin consisting of: (a) pulverizing dried Coleus roots; (b) extracting the root powder obtained in step (a) with a solvent selected from a mixture of water and alcohol, Cl-C4 alcohols, methylene dichloride, toluene, or hexane; (c) concentrating the extract obtained in step (b); (d) precipitating the concentrate obtained in step (c) with a non-polar solvent selected from heptane, pentane or hexane; (e) filtering the precipitate obtained in step (d); (f) back extracting the filtrate obtained in step (e) with a mixture of water and alcohol; and (g) crystallizing the isoforskolin in alcohol. Also disclosed is a commercial process for making isoforskolin consisting of: (a) pulverizing dried Coleus roots; (b) extracting the root powder obtained in step (a) with supercritical carbon dioxide and cosolvent ethanol at a temperature of 45° to 55° C and pressure 300 bar; and (c) crystallizing the isoforskolin in ethanol. Optionally the extract from step (b) can be hydrolysed with a lipase enzyme to form 7-deacetylforskolin and crystallizing the 7-deacetylforskolin in ethanol.</p> |