METHOD FOR PRODUCING CERAMIDE IN TRANSFORMED CELLS USING THE SPINGOLIPID-DELTA4-DESATURASE ATTACHED WITH AN ENDOPLASMIC RETICULUM LOCALIZATION SIGNAL

摘要

The present invention provides a method for producing human ceramide in cells such as yeast cells. The method of the present invention comprises:
1) introducing, by transformation of the yeast cell, an endoplasmic reticulum localization signal-attached sphingolipid ”4-desaturase gene (DES1m) in which a nucleotide sequence encoding a yeast endoplasmic reticulum localization signal is added to the 3'-end of the sphingolipid ”4-desaturase gene (DES1); and
2) abolishing, by transformation of the yeast cell, the expression of the yeast sphinganine C4-hydroxylase gene (SUR2).