PLASTID TRANSFORMATION VECTORS ALLOWING EXCISION OF MARKER GENES

摘要

The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and/or mitochondria and which comprises a selection gene, for example isopentenyl transferase (IPT) and one or more transgenes encoding polypeptides involved in fatty acid biosynthesis, modification or degradation. After selection for transformed plastids and/or mitochondria, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and/or mitochondria and the expression of the transgene in the plastid and/or mitochondria. The invention also provides cells and plants comprising the Transformation Cassette.