MONOCLONAL ANTIBODIES SPECIFIC TO UNDIFFERENTIATED HUMAN EMBRYONIC STEM CELL

摘要

PURPOSE: A monoclonal antibody which specifically binds to an undifferentiated human embryonic stem cell and a hybridoma producing the same are provided to specifically recognize cell surface protein of the undifferentiated human embryonic stem cell and remove undifferentiated embryonic stem cell during cell therapy. CONSTITUTION: A method for preparing monoclonal antibody which is specific to an undifferentiated human embryonic stem cell comprises: a step of injecting differentiated human embryonic stem cells to one side of animal body; a step of injecting undifferentiated human embryonic stem cells to the other side of animal body to induce immunity; and a step of removing antibodies to common surface antigens which exist in both differentiated and undifferentiated embryonic stem cell from lymph node of the other side of the animal body.