Fusion constructs and use of same to produce antibodies with increased Fc receptor binding affinity and effector function

摘要

A method for producing a polypeptide in an isolated yeast or mammalian host cell, comprising, a. culturing an isolated yeast or mammalian host cell engineered to express at least one nucleic acid encoding a polypeptide having a-Mannosidase II activity under in vitro or ex vivo conditions which permit the production of a polypeptide selected from the group consisting of a whole antibody molecule, an antibody fragment, and a fusion protein that includes a region equivalent to the Fc region of an immunoglobulin, wherein said polypeptide having (lMannosidase II activity is expressed in an amount sufficient to modify the oligosaccharides in the Fe region ofsaid polypeptide produced by said host cell; and b. isolating said polypeptide produced by said host cell. Also disclosed are polypeptides, antibodies and fusion proteins produced by the method. The products of the method are useful in treating tumours and diseases associated with B-cell depletion. (62) Divided Out of 541503