发明名称 |
A process for the purification of antibodies involving addition of a second resin |
摘要 |
Disclosed is a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger. |
申请公布号 |
NZ548126(A) |
申请公布日期 |
2009.10.30 |
申请号 |
NZ20050548126 |
申请日期 |
2005.02.25 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
GRONBERG, ANNA;JOHANSSON, BO-LENNART;JOHANSSON, HANS J;MALOISEL, JEAN-LUC;THEVENIN, NICOLAS |
分类号 |
B01D15/08;C07K16/00 |
主分类号 |
B01D15/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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