| 摘要 |
The invention relates to primer-specific and mispair extension assays for identifying gene variations, such as in different genotypes or subtypes of a given genotype. The assay includes extending a nucleic acid sequence from a patient sample with extension products of the primer, characterizing the extension products, and comparing the extension products with known nucleic acid sequences of various genotypes for determining the genotype of the nucleic acid sequence extended. The one or more extension products are terminated in the presence of at least two mispairs within a 2 to 4 base pair range located downstream of the 3' end of the primer. In the assay, at least one primer or the dNTPs is labeled.
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