发明名称 |
Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains |
摘要 |
A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
|
申请公布号 |
US7608437(B2) |
申请公布日期 |
2009.10.27 |
申请号 |
US20050218433 |
申请日期 |
2005.09.06 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
ASAKURA YOKO;NAKAMURA JUN;KANNO SOHEI;SUGA MIKIKO;KIMURA EIICHIRO;ITO HISAO;MATSUI KAZUHIKO;OHSUMI TSUYOSHI;NAKAMATSU TSUYOSHI;KURAHASHI OSAMU |
分类号 |
C12N15/37;C12P13/14;C07H21/00;C07K14/00;C12N1/21;C12N9/02;C12N9/10;C12N15/00;C12N15/67;C12P13/04;C12P21/00;C12Q1/32;C12Q1/48;C12Q1/68 |
主分类号 |
C12N15/37 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|