METHODS FOR ENHANCED PRODUCTION OF BONE MORPHOGENETIC PROTEINS

摘要

Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-ß superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 µM and if pyridoxal is present, it makes up less than 55 % of the molar concentration of vitamin B6 in the media.