发明名称 |
Cell-based assays employing voltage and calcium dyes |
摘要 |
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
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申请公布号 |
US7604959(B2) |
申请公布日期 |
2009.10.20 |
申请号 |
US20070783884 |
申请日期 |
2007.04.12 |
申请人 |
ATTO BIOSCIENCE INC. |
发明人 |
YAO YONG;CAO LIANG;LU JIANMING;LLORENTE ISABEL |
分类号 |
C12N15/12;C07H21/04;C07K14/705;C12N15/09 |
主分类号 |
C12N15/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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