| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of selectively and sensitively detecting nucleic acid, and reliable large-scale screening methods, for a large number of genomic mutations in heterogeneous biological samples. SOLUTION: The invention provides methods of detecting mutation with high sensitivity and high selectivity. In a general embodiment, the invention comprises a single base extension reaction that is repeated at least once. The methods are useful for detecting and identifying genetic mutations or the presence of disease-causing microorganisms in heterogeneous biological samples. COPYRIGHT: (C)2010,JPO&INPIT |
