<p>Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.</p>
申请公布号
EP2108042(A2)
申请公布日期
2009.10.14
申请号
EP20080728823
申请日期
2008.02.01
申请人
CALIFORNIA INSTITUTE OF TECHNOLOGY;STANFORD UNIVERSITY;UNIVERSITY OF CHICAGO
发明人
SCHWARTZ, JERROD;QUAKE, STEPHEN, R.;MRKSICH, MILAN