发明名称 |
Reverse two-hybrid systems |
摘要 |
Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.
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申请公布号 |
US7601533(B2) |
申请公布日期 |
2009.10.13 |
申请号 |
US20060352283 |
申请日期 |
2006.02.13 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;THE JOHN HOPKINS UNIVERSITY |
发明人 |
VIDAL MARC;BOEKE JEF;HARLOW ED |
分类号 |
C12N15/09;C12N15/85;C12N1/19;C12N15/10;C12N15/63;C12Q1/68;C12R1/865;G01N33/50;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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