发明名称 |
GENERATION OF TUMOR-FREE EMBRYONIC STEM-LIKE PLURIPOTENT CELLS USING INDUCIBLE RECOMBINANT RNA AGENTS |
摘要 |
The present invention generally relates to a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells. More particularly, the present invention relates to a method and composition for generating a Tet-On/Off recombinant transgene capable of expressing a manually re-designed mir-302 microRNA (miRNA)/shRNA agent under the control of doxycyclin (Dox) in human somatic/cancer cells and thus inducing certain specific gene silencing effects on the differentiation-associated genes and oncogenes of the cells, resulting in reprogramming the cells into an ES-like pluripotent state. |
申请公布号 |
WO2009091659(A3) |
申请公布日期 |
2009.10.08 |
申请号 |
WO2009US30432 |
申请日期 |
2009.01.08 |
申请人 |
LIN, SHI-LUNG;WU, DAVID TS |
发明人 |
LIN, SHI-LUNG;WU, DAVID TS |
分类号 |
C12N5/074;C12N15/113 |
主分类号 |
C12N5/074 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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