METHOD FOR FLUORESCENCE ANALYSIS AND FLUORESCENCE ANALYZER

摘要

The present invention aims at reducing background noise derived from a substance that is present in the vicinity of a target substance such as a DNA and protein and attached to the surface of a substrate without an effect on a fluorescent dye labeling the target substance. The substrate that has a probe and is capable of interacting with the target substance is irradiated with noise removing light such that an evanescent field is generated on the surface of the substrate. A target substance and a foreign particle, which are non-specifically stuck to the surface of the substrate, are decomposed by the evanescent field generated by the irradiation with the noise removing light. The evanescent field present near the surface of the substrate has almost no effect on the probe. It is possible to reduce the background noise derived from the substance that is present in the vicinity of the target substance and attached to the surface of the substrate and suppress effects on the probe and the target substance interacting with the probe.