METHOD FOR INDUCTION OF THE DIFFERENTIATION OF VISCERAL FAT CELL

摘要

A culture medium is disclosed for inducing the differentiation of visceral preadipocytes into mature visceral adipocytes; the culture medium contains 0.85 to 100 ng/mL insulin and 50 to 250 ng/mL IGF-1. Also disclosed is a method for using the culture medium to induce the differentiation of visceral preadipocytes into mature visceral adipocytes. Use of the differentiation induction system of the present invention enables a substantial induction of adipocyte differentiation without the addition of synthetic differentiation inducers or high insulin concentrations. The mature adipocytes obtained by the differentiation induction system of the present invention are useful for research into the biochemistry and physiology of adipocytes, for screening drugs effective for the treatment of lifestyle-related diseases such as obesity and type 2 diabetes, and for developing diagnostic reagents.