METHOD FOR CREATING BROAD-SPECTRUM RESISTANCE TO FUNGI IN TRANSGENIC PLANTS

摘要

The present invention relates to the creation of broad-spectrum resistance in transgenic plants by incorporating inhibitory nucleic acid sequences, which inhibit the expression of fungus genes. The nucleic acid sequences of genes critical to the development, growth and multiplication of fungi, which is to say sequences of genes having an essential function in fungi, are often preserved, or have high sequence identity. Regions having particularly high sequence identity between various fungi are used to produce inhibitory gene constructs, such as on the basis of antisense, siRNA, shRNA, ribozyme technology and other technologies, which impart the inhibition of the expression and activation of genes. Since these methods are based on the sequence-specific hybridization of the inhibitory RNA molecules having the corresponding target sequences in certain fungus genes, the expression of all corresponding genes from various fungi is therefore also inhibited. Due to the high sequence identity of such preserved gene sections, transgenic plants are produced, which have broad-spectrum resistance to various fungi.