INTERNAL POSITIVE CONTROL FOR NUCLEIC ACID ASSAYS

摘要

Compositions and methods for detecting a non-specific nucleic acid amplification inhibitor in a reaction are disclosed. An internal positive control (IPC) may be included in samples to be tested for target nucleic acids as a means to monitor non-specific inhibition of nucleic acid amplification and provide confidence in negative results obtained in target-specific assays. Provided herein are an IPC polynucleotide, IPC control primers, and IPC probes. Also provided are methods of using an IPC polynucleotide, primers, and probes to detect a non-specific nucleic acid amplification inhibitor.