METHOD OF PRODUCING SINGLE-STRANDED DNA

摘要

The invention relates to a method for producing single-stranded DNA and to a kit for use in said method. The method comprises a first PCR stage comprising repeating denaturation, annealing and extension steps, and a second PCR stage comprising denaturation and combined annealing and extension steps. In the method, at least one first and at least one second primer having partly- overlapping annealing temperature areas are used. Thus, the amplification takes place by thermal asymmetric PCR.