Serum-free cell culture medium for mammalian cells

摘要

Provided is a process for the production of growth hormone comprising the step of culturing cells of a cell line expressing growth hormone in a cell culture medium free of components derived from animal serum, the medium comprising Zinc in a concentration ranging from 0.2 microM to 1.75 microM and Copper in a concentration ranging from 10 nM to 75 nM and Ferric ions in a concentration ranging from 1 to 10 microM. Further provided are similar processes with specified media and promoters controlling expression and use of the media for the production of growth hormone.