| 摘要 |
Disclosed are isolated polynucleotide sequences from several Lactobacillus species, including L. acidophilus, L. brevis, L. casei, and L. delbrueckii. All sequences relate to CRISPR loci, which are repetitive regions that are highly conserved within species, but show low similarity between species. In particular the CRISPR region of L. acidophilus is disclosed. Further disclosed are methods and kits for the detection and typing of Lactobacillus bacterial strains from in vitro samples, food products, dietary supplements, and environmental samples, primarily using the CRISPR sequence of L. acidophilus as a reference. |
