| 摘要 |
A microarray for the analysis of single-stranded nucleotide sequences, in particular miRNAs, comprising a plurality of probe strands (5) bound to a substrate (2) and adapted to form respective probe hybrids (1) with a labelling segment (3) bound to a fluorophore moiety (4) and with a ligating segment. Each of the probe strands (5) consists of a complementary ligating sequence hybridised in use to the ligating segment, a complementary labelling sequence (8) hybridised in use to the labelling segment (3), and a probe sequence (7) positioned between the complementary ligating sequence and the complementary labelling sequence (8) and adapted to selectively ligate a target single-stranded nucleotide sequence.
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