Improvements in thermal cycler for PCR

摘要

A method for calibrating a removable assembly for cycling samples through a series of temperature excursions is disclosed. The removable assembly is attached to an instrument and comprises a sample block for receiving vials, a plurality of thermoelectric devices, a heat sink, a clamping mechanism positioned so as to clamp the thermoelectric devices between the sample block and the heatsink, and a memory device capable of storing data related to the removable assembly. The method comprises the steps of: applying power to the thermoelectric devices, causing the removable assembly to cycle through a desired series of temperature excursions, measuring the actual temperature excursions, comparing the actual temperature excursions with the desired temperature excursions, calibrating the removable assembly by adjusting the power applied to the thermoelectric devices so that the actual temperature excursions match the desired temperature excursions and recording the adjusted power in the memory device for further utilization in obtaining the desired series of temperature excursions, to form a calibrated removable assembly, removing the calibrated removable assembly from the instrument, and attaching the removable assembly to a different instrument.