| 摘要 |
<p>The present invention relates to a double-stranded probe intended for the fluorescent detection of at least one single-stranded or double-stranded target nucleic acid, comprising:
- a first strand of formula X 1 -(L 1 ) a -S 1 -S' 1 -(L' 1 ) b -Y 1 intended for the detection of a first strand of the target nucleic acid which comprises a sequence of formula T' 1 -T 1 ;
- a second strand of formula X 2 -(L 2 ) c -S 2 -S' 2 -(L' 2 ) d -Y 2 intended for the detection of a second strand of the target nucleic acid, if present, said second strand of the target nucleic acid comprising a sequence of formula T' 2 -T 2 ;
wherein two of X 1 , X 2 , Y 1 , and Y 2 represent a fluorescent donor, while the two others represent a fluorescent acceptor, and X 1 and Y 2 can not both represent a fluorescent donor.</p> |
