A bio chip is provided to analyze concrete ingredient of bio sample by observing the reaction between a probe and bio sample and read various date. A bio chip comprises a substrate(11) and probe(200). The substrate comprises plural surface protrusions(50). The plurality of probes are formed by coupling by each the surface protrusion. The surface protrusion and substrate is in one form. The probe is classified into non-prove cell area which does not form coupling and probe cell area which forms coupling. The surface protrusion is formed at the plural probe cell areas.
