METHOD FOR DETECTION OF NUCLEIC ACID BARCODES

摘要

A method of sample analysis is provided. In certain embodiments, the method may comprise: a) contacting a surface-tethered oligonucleotide with a sample comprising a barcode oligonucleotide to produce an oligonucleotide duplex comprising a double-stranded surface-proximal region and a single-stranded surface-distal overhang; b) extending the barcode oligonucleotide using the overhang as a template to produce an extended duplex; c) subjecting the extended duplex to a wash that separates the oligonucleotide duplex but does not separate said extended duplex; and d) detecting the extended duplex.