The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.
申请公布号
WO2009085333(A1)
申请公布日期
2009.07.09
申请号
WO2008US62647
申请日期
2008.05.05
申请人
GE HEALTHCARE BIO-SCIENCES CORP.;MITSIS, PAUL;SEKHER, ANURADHA;KUMAR, GYANENDRA