MICROWAVE ANTIGEN RETRIEVAL IN NON-AQUEOUS SOLVENTS

摘要

The present disclosure provides methods for high temperature antigen retrieval for immunohistochemistry. In particular examples, the method includes contacting a sample including an antigen that has been fixed by a cross-linking agent with an antigen retrieval solution including a non-aqueous solvent, and heating the antigen retrieval solution to a temperature of 100° C to 200° C using microwave energy. In some examples, the antigen retrieval solution includes at least one polar solvent or ionic liquid.