发明名称 |
Enhancement of immunochemical staining in aldehyde-fixed tissue |
摘要 |
A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
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申请公布号 |
USRE40835(E1) |
申请公布日期 |
2009.07.07 |
申请号 |
US19930249180 |
申请日期 |
1993.08.12 |
申请人 |
BIOGENX LABORATORIES, INC. |
发明人 |
SHI SHAN-RONG;TANDON ATUL K.;KALRA KRISHAN L.;MALHOTRA NAGESH;SU SHENG-HUI;YU CHENG-ZHI |
分类号 |
G01N33/567;G01N1/30;G01N33/53 |
主分类号 |
G01N33/567 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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