CELL-BASED REPORTER SYSTEM FOR SCREENING NATURAL PRODUCTS REACTIVATING GAMMAHERPESVIRUS AND SCREENING METHOD USING THE SAME

摘要

A reporter system for searching a natural compound reactivating gamma herpesvirus in cell line infected with gamma-herpesvirus is provided to help activation of anti-herpes agent and search natural compound without toxicity. A reporter cell line for searching an inducing material of reactivating gamma herpes virus comprises: a PAN RNA (polyadenylated nuclear RNA) promoter (sequence number 1[SEQ ID NO:1]) of Kaposi's sarcoma-associated herpesvirus (KSHV); and a reporter gene which is connected to be expressed by a promoter. The reporter gene is destabilized enhanced green fluorescent protein (EGFP). The gamma herpes virus is a cell line which is infected with KSHV or Epstein-Barr virus (EBV).