PRIMERS FOR PCR AMPLIFICATION COMPRISING ABASIC PARTS WITHIN THE PRIMER SEQUENCES
摘要
The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.
申请公布号
WO2009045067(A3)
申请公布日期
2009.07.02
申请号
WO2008KR05821
申请日期
2008.10.02
申请人
BIONEER CORPORATION;KIM, HYUN BAE;KIM, SEONG YOUL;GIL, JUN MO;PARK, HAE JOON;PARK, HAN OH
发明人
KIM, HYUN BAE;KIM, SEONG YOUL;GIL, JUN MO;PARK, HAE JOON;PARK, HAN OH