| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a labeled polynucleotide. <P>SOLUTION: A substituted propargylethoxyamido nucleoside with a structure represented by figure (I) is disclosed, in which X is selected from the group consisting of an aminoalkanoic acid, an alkylaminobenzoic acid,α-benzoic acid, and 4-amino-2-butynic acid; R<SB>1</SB>and R<SB>2</SB>are each individually selected from the group consisting of -H, a lower alkyl group, a protective group, and a label; R<SB>3</SB>is selected from the group consisting of -H and a lower alkyl group; and B is 7-deazapurine, purine, or a pyrimidine nucleoside base. In addition, a primer extension method is provided, which uses the above X-substituted propargylethoxyamido nucleoside, and a polynucleotide containing the above X-substituted propargylethoxyamido nucleoside is provided. <P>COPYRIGHT: (C)2009,JPO&INPIT</p> |
