METHOD AND APPARATUS FOR SINGLE SIDE BILAYER FORMATION
摘要
An apparatus for single-sided bilayer formation includes a first fluid chamber including a sidewall and a second fluid chamber extending through the sidewall. A barrier wall separates the first and second fluid chambers and includes a nanopore therein across which a planar lipid bilayer (PLB) is formed. In use, an electrolyte is added to the first and second fluid chambers and a lipid/organic solvent mixture is added to the first fluid chamber to form a lipid/organic solvent layer. The electrolyte level within the first fluid chamber is adjusted such that the lipid layer is raised above the barrier wall and a PLB is formed. Electrolyte levels may be adjusted manually or utilizing a fluid level regulator with or without feedback control. The apparatus may be in a nanopore array and may be incorporated into an ion channel sensing system.
申请公布号
WO2009073622(A1)
申请公布日期
2009.06.11
申请号
WO2008US85174
申请日期
2008.12.01
申请人
ELECTRONIC BIO SCIENCES, LLC;ERVIN, ERIC, N.;BARRALL, GEOFFREY, ALDEN