发明名称 |
EXPRESSION CASSETTE FOR LACTASE DEHYDROGENASE, TRANSFORMED YEAST AND METHOD OF PRODUCING LACTIC ACID |
摘要 |
Disclosed are an expression cassette for a gene encoding lactate dehydrogenase, which is required for preventing decreases in lactic acid yield and lactic acid production speed in continuously culturing a yeast capable of producing lactic acid under filtration through a separation membrane so that a high optical purity, a high lactic acid yield and a high lactic acid production speed can be achieved at the same time, a yeast having the preceding cassette, and a method of producing lactic acid by culturing the preceding yeast. The expression cassette for lactate dehydrogenase as described above is an expression cassette for lactate dehydrogenase having a gene encoding lactate dehydrogenase which is ligated to the downstream of a promoter, wherein the above-described promoter is a promoter of a gene which shows, in continuously culturing a yeast capable of producing lactic acid under filtration through a separation membrane, a gene expression amount that is 5 times or more larger than the average of the relative expression amounts of all genes 50 hours or later after the start of the culture. |
申请公布号 |
WO2009072593(A1) |
申请公布日期 |
2009.06.11 |
申请号 |
WO2008JP72129 |
申请日期 |
2008.12.05 |
申请人 |
TORAY INDUSTRIES, INC.;SAWAI, KENJI;SAWAI, HIDEKI |
发明人 |
SAWAI, KENJI;SAWAI, HIDEKI |
分类号 |
C12N15/09;C12N1/19;C12P7/56 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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