| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new antigen construct useful as a reagent in immunoassay for detection and identification of antibodies to human immunodeficiency virus type 1 (HIV-1) group M, HIV-1 group O and human immunodeficiency virus type 2 (HIV-2). <P>SOLUTION: Isolated HIV-1 group O env polypeptides obtained from the HIV-1 isolate HAM112 are provided, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 group O env polypeptides and HIV-1 group M env polypeptide and (b) further antigen constructs containing additional group O sequences (especially the gp41 IDR of isolate HAM112). Also polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs are provided. <P>COPYRIGHT: (C)2009,JPO&INPIT |
