摘要 |
<P>PROBLEM TO BE SOLVED: To provide a DNA and vector having engineered recombination sites for use in recombinational cloning enabling an effective and specific recombination of a DNA segments using a recombination protein. <P>SOLUTION: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s). <P>COPYRIGHT: (C)2009,JPO&INPIT |