发明名称 |
Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest |
摘要 |
The present invention relates to a method of expressing an objective protein at a high level and stably as well as for a long period even in the absence of a selection drug with a recombinant mammal cell. More particularly, the present invention relates to a method of producing an objective protein by providing a recombinant mammal cell having multiple copies of the exogenous objective protein gene expression unit integrated into a hypoxanthine-phosphoribosyl transferase enzyme (hprt) gene locus and culturing said cell.
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申请公布号 |
US2009117615(A1) |
申请公布日期 |
2009.05.07 |
申请号 |
US20080222412 |
申请日期 |
2008.08.08 |
申请人 |
BANZAI TOSHIAKI;KOYAMA YUKIKO;SONEZAKI SHUJI |
发明人 |
BANZAI TOSHIAKI;KOYAMA YUKIKO;SONEZAKI SHUJI |
分类号 |
C12P21/00;C12N5/10;C12N15/09 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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