Method and apparatus for continuous large-scale radiolabeling of proteins

摘要

Disclosed are improved methods and apparatus for radiolabeling, particularly methods and apparatus for large scale in-line radiolabeling of products, such as proteins and antibodies. Particularly, A continuous mode radiolabeling apparatus for labeling a molecule with a radioactive label, said apparatus comprising: a reaction chamber or tube comprising a first end and a second end; a first reagent vessel fluidly connected to said first end of said reaction chamber or tube through a first reagent tubing, said first reagent tubing connected to at least a first pump; a second reagent vessel fluidly connected to said first end of said reaction chamber or tube through a second reagent tubing, said second reagent tubing connected to a pump; a radioshield, wherein at least one of said reagent vessels is comprised within said radioshield; and at least a first purification unit comprising an inlet and an outlet, said inlet of said purification unit fluidly connected to said second end of said reaction chamber or tube.