Single-step, highly sensitive yet specific and quantitative method to detect and quantify endothelial progenitor cells (EPCs) in human peripheral blood

摘要

Disclosed is a synthetic apolipoprotein-E mimicking polypeptide comprising an amino acid sequence selected from the group of (i) X-Y-Arg-Arg-Y-Y-X-X-Y-Y-Arg-Y-Y-Arg-X-Y-Y-X or the reverse sequence thereof, (ii) Arg-Arg-Y-Y-X-X-Y-Y-Arg-Y-Y-Arg-X-Y or the reverse sequence thereof, (iii) Y-Y-X-X-Y-Y-Arg-Y-Y-Arg-X-Y-Y-X or the reverse sequence thereof, and (iv) X-Y-Arg-Arg-Y-Y-X-X-Y-Y-Arg-Y-Y-Arg or the reverse sequence thereof, wherein X is glycine, threonine, serine or alanine, wherein Y is a hydrophobic amino acid, wherein the polypeptide comprises an acetyl group at the N-terminus and an amide group at the C-terminus, and wherein the polypeptide consists of a single domain.