| 摘要 |
The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems. According to the method of the present invention, glycosyltransferases derived from microorganisms of the Vibrionaceae, such as beta-galactoside-alpha2,6-sialyltransferase derived from Photobacterium damselae, beta-galactoside-alpha2,3-sialyltransferase derived from Photobacterium phosphoreum and beta-galactoside-alpha2,3-sialyltransferase derived from Vibrio sp., enhance their enzymatic activity when an appropriate amount of NaCl is added to their enzyme reaction systems.
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