发明名称 |
HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION |
摘要 |
Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers and oligomeric and monomeric substrate constructs are also disclosed. |
申请公布号 |
WO2008157696(A3) |
申请公布日期 |
2009.04.02 |
申请号 |
WO2008US67507 |
申请日期 |
2008.06.19 |
申请人 |
STRATOS GENOMICS INC.;KOKORIS, MARK, STAMATIOS;MCRUER, ROBERT, N. |
发明人 |
KOKORIS, MARK, STAMATIOS;MCRUER, ROBERT, N. |
分类号 |
C12Q1/68;C07H21/02;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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