发明名称 |
PROCESS AND GENES FOR EXPRESSION AND |
摘要 |
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
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申请公布号 |
US2009087880(A1) |
申请公布日期 |
2009.04.02 |
申请号 |
US20060573035 |
申请日期 |
2006.02.28 |
申请人 |
SEIBERT MICHAEL;KING PAUL W;GHIRARDI MARIA LUCIA;POSEWITZ MATTHEW C;SMOLINSKI SHARON L |
发明人 |
SEIBERT MICHAEL;KING PAUL W.;GHIRARDI MARIA LUCIA;POSEWITZ MATTHEW C.;SMOLINSKI SHARON L. |
分类号 |
C12P21/04;C12N1/21 |
主分类号 |
C12P21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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