A GENE CLUSTER ENCODING ANTIBIOTIC ACTIVITY FROM SOIL METAGENOME

摘要

A gene cluster encoding antibiotic activity from metagenome of soil microbes is provided to produce antibiotic by transforming Escherichia coli, which has antifungal activity and antibacterial activity. A gene cluster encoding antibiotic activity includes orf20 to orf39 obtained from metagenome. A gene cluster from orf29 to orf36 directly involves expression of antibiotic activity. A gene cluster from orf20 to orf28 controls the expression of antibiotic activity genes. Proteins encoded by antibiotic activity genes from orf29 to orf36 have amino acid sequence of sequence number 4 to the sequence number 11. Proteins encoded by antibiotic activity genes from orf20 to orf28 have amino acid sequence of sequence number 2 and the sequence number 3.