发明名称 |
PORTABLE, TEMPERATURE AND CHEMICALLY INDUCIBLE EXPRESSION VECTOR FOR HIGH CELL DENSITY EXPRESSION OF HETEROLOGOUS GENES IN ESCHERICHIA COLI |
摘要 |
<p>The present disclosure relates to nucleic acids comprising a sequence of SEQ ID NO: 1. The nucleic acid may be an isolated DNA and/or may be in the form of a plasmid or an expression vector. It may also be comprised in a microorganism. The nucleic acid may further comprise sequences that encode a protein. The self-replicating expression plasmid comprising a DNA sequence of the disclosure may be used to produce one or more protein. The production of one or more protein by a plasmid of the disclosure may be controlled by temperature and/or chemical induction. The disclosure also provides methods of obtaining high yields of proteins and methods for purifying such proteins, such as the LdK39 protein or a fragment thereof.</p> |
申请公布号 |
WO2009038841(A2) |
申请公布日期 |
2009.03.26 |
申请号 |
WO2008US66742 |
申请日期 |
2008.06.12 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM;BRANDIS, JOHN W.;JOHNSON, KENNETH A. |
发明人 |
BRANDIS, JOHN W.;JOHNSON, KENNETH A. |
分类号 |
C12P21/06 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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