| 摘要 |
The present invention relates to the field of veterinary medicine, more specifically to compositions and methods useful for processing animal semen for use in assisted reproduction. The invention relates to a composition for separation of spermatozoa from a semen sample, comprising at least one salt of an alkali metal and/or an alkaline earth metal, EDTA, a zwitterion buffer, silane-coated silica particles and water, said composition having a pH of 7.0- 7.35 and an osmolarity of 300-345 mOsm. Furthermore it relates to a method for preparing spermatozoa from a semen sample from a non-human animal, comprising the step of separating the spermatozoa from other semen constituents by centrifugation through a single layer of a colloid formulation. Preferably, the colloid formulation is the composition according to the invention. The invention also relates to a method for separating a sperm sub-population of interest from a semen sample from a non-human animal, comprising the steps of providing a density gradient comprising at least two layers of the composition of the invention, each layer having a different density;separating the sperm sub-populations in the semen sample by centrifugation through the density gradient; and selecting the sperm sub-population of interest. Finally, the invention relates to spermatozoa prepared with the methods of the invention and the use of such spermatozoa in artificial insemination, in vitro fertilisation or intracytoplasmic sperm injection. |
