METHOD OF CONCENTRATING HUMAN MESENCHYMAL STEM CELLS

摘要

It is intended to provide a method of highly concentrating human mesenchymal stem cells from a mass of cells containing the human mesenchymal stem cells. To highly concentrate human mesenchymal stem cells, CD271+CD90+ cells are collected from a mass of cells containing the human mesenchymal stem cells by using flow cytometry or the like. In the case where the mass of cells contains blood cells (for example, a mass of cells prepared form bone marrow, peripheral blood, etc.), CD45-CD235a-CD271+CD90+ cells are collected. These cell fractions contain highly pure mesenchymal stem cells that have self-renewal capability, self-propagating capability and pluripotency. Therefore, human mesenchymal stem cells can be highly concentrated by collecting the CD271+CD90+ cells from the mass of cells containing the human mesenchymal stem cells.