A method for preparing a stable immunogenic product comprising antigenic heterocomplexes of TNFalpha and a carrier protein

摘要

Disclosed is a method for preparing an anti-TNFalpha immunogenic product, comprising the steps of: a) providing a liquid solution containing TNFalpha; b) adding EDTA to said liquid solution containing TNFalpha of step a); c) adding a carrier protein to the liquid solution obtained at the end of step b); d) adding glutaraldehyde to the liquid mixture obtained at the end of step c); e) removing glutaraldehyde and free molecules of both TNFalpha and said carrier protein from the solution obtained at the end of step d); f) adding formaldehyde to the liquid solution obtained at the end of step e), and maintaining the presence of formaldehyde for a period of time ranging from 96 hours to 192 hours; g) adding a reagent blocking the reaction with formaldehyde to the liquid solution obtained at the end of step f); and h) removing formaldehyde and the blocking reagent from the liquid solution obtained at the end of step h), so as to obtain a liquid solution containing said anti-TNFalpha immunogenic product. Also disclosed are products made by said method.