AN ISOLATED DNA FRAGMENT OF THE HUMAN A33 PROMOTER AND ITS USE TO CONTROL THE EXPRESSION OF A HETEROLOGOUS GENE IN TUMOR CELLS

摘要

An isolated DNA sequence that corresponds to a region of the human A33 gene promoter from base pair −105 to base pair +307, able to control the expression of a heterologous gene of interest, that may be used in conjunction with any other regulatory sequence, including sequences responsive to stress such as radiation, hypoxia and free radical formation. Constructs and viral vectors for recombinant DNA expression are provided, which include this sequence of human A33 gene promoter and, operably linked thereto, at least one heterologous gene. The regulatory sequence(s) control(s) the expression of at least one heterologous gene in cells including colorectal cancer cells. Pharmaceutical compositions and methods for treating colorectal cancer are also described.