UMPS ISOFORMS AS NOVEL PREDICTIVE MARKERS AND THERAPEUTIC TARGETS FOR CHEMOTHERAPEUTICS

摘要

a method of predicting a subject's response to a chemotherapeutic treatment comprising the steps of: obtaining a sample from the subject, detecting a target gene in the sample, and assessing sample to detect at least one isoform of the target gene. if an isoform is detected, then identifying and quantifying isoform. the quantified isoform is compared to a suitable control. a change in expression of the quantified isoform relative to the control indicates the subject's responsiveness to said chemotherapeutic treatment. one embodiment relates to assessing a subject's responsiveness to chemotherapeutic treatments comprising 5-fluorouracil, by detecting, assessing and quantifying selected isoforms of uridine 5'-monophosphate synthase gene. another embodiment relates to assessing a subject's responsiveness to chemotherapeutic treatments comprising 5-fluorouracil, by detecting, assessing and quantifying isoforms a and b, and determining the ratio of expression of isoforms a to b compared to the ratio of expression of a suitable control.